Complimentary Webinar Recording Available
Originally Presented during ESHRE 2019 in Vienna
Title: Continuous Flow of Pre-Mixed Gas is not Required to Maintain Culture Medium pH in a Benchtop Incubator
Presenter: Dr. David Mortimer
BCSI is excited to invite you to join Dr. David Mortimer for a complimentary encore webinar presentation of his 2019 ESHRE oral abstract entitled, “Continuous Flow of Pre-Mixed Gas is not Required to Maintain Culture Medium pH in a Benchtop Incubator.” The initial presentation is also followed by a live discussion with webinar participants.
About Dr. Mortimer:
Originally from the UK: BSc (Hons) Zoology from Bristol (UK) 1974 and PhD (Edinburgh) 1977; post-doctoral fellowships in Edinburgh, Paris and Birmingham; Faculty member at the University of Calgary (Canada) in Obs & Gynae and Medical Physiology 1983–1991. Research focussed on human sperm pathophysiology. Scientific Director of Sydney IVF (Australia) 1991–1999. Co-founder of the ESHRE Basic Semen Analysis courses in 1994. President of the Canadian Fertility and Andrology Society 2009–2010; Associate Editor of Human Reproduction Update 2011–2014. Member of Accreditation Canada’s ART Standards Working Group since 2007, and of the Canadian Standards Association since 2008. Honorary Senior Lecturer, University of Dundee School of Medicine (UK) since 2017.
David has given many invited lectures and taught in workshops and courses worldwide. Author/co-author of almost 300 conference presentations and more than 150 publications, including the books Practical Laboratory Andrology (Oxford University Press, 1994), Quality and Risk Management in the IVF Laboratory (Cambridge University Press, 2005; 2nd edition 2015), and A Practical Guide to Basic Laboratory Andrology (Cambridge University Press, 2010). Academic publications h-index = 38.
He became a full-time consultant in 1999 and, with his wife Dr Sharon Mortimer, established the Vancouver-based international reproductive biomedicine consulting company, Oozoa Biomedical, in 2000. Major activities include designing new ART laboratories and cryobanks, upgrading or implementing new lab systems, helping laboratories develop Total Quality Management programmes, performing TQM audits, and advising companies developing products for the reproductive biomedicine field.
Currently he is the Scientific Advisor to Eden Fertility Management Group (Pasadena, CA, USA), Scientific Director for the Sidra Reproductive Medicine Unit (Doha, Qatar), and Executive Scientific Director for the Vitalab Centre for Assisted Conception (Sandton, Gauteng, South Africa); he also serves on Scientific Advisory Panels for Mellowood Medical (Canada), Planer (UK), Hamilton Thorne (USA) and SCSA Diagnostics (USA).
Check Out Our Other Webinars
AAB 2019 Oral Presentation Webinar
In this webinar, BCSI Embryology Product Specialist Susan Olds covers her Oral Presentation, “Workflow Patterns Effect pH Variance in Microdroplet Culture Systems” originally presented at the 2019 AAB Conference in New Orleans.
- Topics Covered Include:
- Review of pH Fundamentals
- pH Monitoring and Our Prototype pH Sensing Microdroplet Dish
- pH Trending And Frequent Use In Cabinet Style Incubator
- pH Map of a Cabinet Style Incubator
- Low Disturbance vs. High Disturbance Testing in a Cabinet Incubator
- pH Trending And Frequent Use in Benchtop Incubators
- Dry Incubator
- Humid Incubator
- Conclusions and Open Discussions
pH Monitoring in Embryo Culture Conditions
During this webinar, we shared some of these pH findings in order to better demonstrate how incubator equipment and common lab operations play a role in maintaining pH in media samples.
Embryology Specialist Susan Olds discusses lab results and examples of pH profiles, both in large format and benchtop (humidified and non-humidified) incubators. The webinar is concluded with a Q&A session at the end.
Real World Best Practices in IVF Cell Culturing- Continuous pH monitoring
Presented by Klaus Wiemer PhD, Lab Director of POMA Fertility Clinic in Kirkland, Washington and Steven Geelhood, SAFE Sens® Project Manager.
Klaus Wiemer PhD discusses the benefits of continuous non-invasive pH monitoring and shares clinical practices that were optimized by POMA Fertility as a result of their utilization of the SAFE Sens system within their incubators including:
- Longer equilibration times for dish preparation
- Increased oil overlay to prevent evaporation
Steven Geelhood (SAFE Sens Project Manager) then shared data collected from labs utilizing the SAFE Sens continuous pH monitory system. Graphs were presented and analyzed for the following scenarios wherein cell culture conditions were manipulated thus causing changes to the pH over time.
- Initial equilibration of media with time scale
- Equilibrated media with or without oil
- CO2 set point changes
- Effect of loss of CO2
Susan Olds
Embryology Product Specialist
Blood Cell Storage, Inc.
Tel: +1.425.654.8462 (D)
Email: susan.olds@safesens.com